A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.
This report has been generated by the nf-core/checkatlas analysis pipeline. For information about how to interpret these results, please see the documentation.
Report
generated on 2023-07-22, 01:19
based on data in:
/data/analysis/data_becavin/checkatlas_test/tuto/work/9c/2553b551000879f5f2747f4fc4b036
Checkatlas Qc Report
Checkatlas Umap Report
CheckAtlas
CheckAtlas A one-liner tool for quality control of your single-cell atlases.
Atlas overview
Overview of your single-cell atlases
Sample Name | AtlasFileType | NbCells | NbGenes | AnnData.raw | AnnData.X | File_extension | File_path |
---|---|---|---|---|---|---|---|
B-cells_compartment | AnnData | 54934.0 | 36503.0 | True | True | B-cells_compartment | /data/analysis/data_becavin/checkatlas_test/tuto/data1/B-cells_compartment.h5ad |
Fetal | AnnData | 27203.0 | 32828.0 | True | True | Fetal | /data/analysis/data_becavin/checkatlas_test/tuto/data1/Fetal.h5ad |
Tabula_Sapiens_Endothelial | AnnData | 31691.0 | 58559.0 | True | True | Tabula_Sapiens_Endothelial | /data/analysis/data_becavin/checkatlas_test/tuto/data1/Tabula_Sapiens_Endothelial.h5ad |
QC total_counts
QC of your atlases log10(Cellrank vs total-counts.
QC n_genes_by_counts
QC of your atlases log10(Cellrank vs n_genes_by_counts.
QC pct_counts_mt
QC of your atlases log10(Cellrank vs pct_counts_mt.
Classification metrics
Quality control metrics calculated on your atlases.
Sample Name | obs | davies_bouldin |
---|---|---|
B-cells_compartment_Manually_curated_celltype | Manually_curated_celltype | 1.3 |
B-cells_compartment_cell_type | cell_type | 1.1 |
B-cells_compartment_cell_type_ontology_term_id | cell_type_ontology_term_id | 1.1 |
Fetal_author_cell_type | author_cell_type | 2.1 |
Fetal_cell_type | cell_type | 1.9 |
Fetal_cell_type_ontology_term_id | cell_type_ontology_term_id | 1.9 |
Fetal_orig.ident | orig.ident | 23.9 |
Fetal_seurat_clusters | seurat_clusters | 2.0 |
Tabula_Sapiens_Endothelial_cell_type | cell_type | 4.5 |
Tabula_Sapiens_Endothelial_cell_type_ontology_term_id | cell_type_ontology_term_id | 4.5 |
Annotation metrics
Quality control metrics calculated on your atlases.
Sample Name | Reference | obs | rand_index |
---|---|---|---|
B-cells_compartment_cell_type | Manually_curated_celltype | cell_type | 1.0 |
B-cells_compartment_cell_type_ontology_term_id | Manually_curated_celltype | cell_type_ontology_term_id | 1.0 |
Fetal_cell_type | author_cell_type | cell_type | 0.7 |
Fetal_cell_type_ontology_term_id | author_cell_type | cell_type_ontology_term_id | 0.7 |
Fetal_orig.ident | author_cell_type | orig.ident | 0.1 |
Fetal_seurat_clusters | author_cell_type | seurat_clusters | 0.4 |
Tabula_Sapiens_Endothelial_cell_type_ontology_term_id | cell_type | cell_type_ontology_term_id | 1.0 |
Dimensionality reduction metrics
Quality control metrics calculated on your atlases.
Sample Name | obsm |
---|---|
B-cells_compartment_X_umap | X_umap |
Fetal_X_pca | X_pca |
Fetal_X_umap | X_umap |
Tabula_Sapiens_Endothelial_X_pca | X_pca |
Tabula_Sapiens_Endothelial_X_scvi | X_scvi |
Tabula_Sapiens_Endothelial_X_scvi_umap | X_scvi_umap |
Tabula_Sapiens_Endothelial_X_umap | X_umap |
Atlas object explorer
Exploration of your Atlas object (Scanpy, Cellanger, Seurat)
Sample Name | atlas_obs | obsm | var | varm | uns |
---|---|---|---|---|---|
B-cells_compartment | donor_id Predicted_labels_CellTypist Majority_voting_CellTypist Manually_curated_celltype assay_ontology_term_id cell_type_ontology_term_id development_stage_ontology_term_id disease_ontology_term_id self_reported_ethnicity_ontology_term_id is_primary_data organism_ontology_term_id sex_ontology_term_id tissue_ontology_term_id suspension_type cell_type assay disease organism sex tissue self_reported_ethnicity development_stage | X_umap | gene_symbols feature_is_filtered feature_name feature_reference feature_biotype |
| cell_type_ontology_term_id_colors default_embedding development_stage_ontology_term_id_colors schema_version sex_ontology_term_id_colors title |
Fetal | orig.ident nCount_RNA nFeature_RNA seurat_clusters donor_id Stage Selection compartment age CnT DTLH UBCD SSBpr End MSC RVCSB SSBpod SSBm.d ICa ErPrT ICb NPC Pod annotCell author_cell_type cell_type_ontology_term_id disease_ontology_term_id self_reported_ethnicity_ontology_term_id development_stage_ontology_term_id tissue_ontology_term_id sex_ontology_term_id organism_ontology_term_id is_primary_data assay_ontology_term_id suspension_type cell_type assay disease organism sex tissue self_reported_ethnicity development_stage | X_pca X_umap | vst.mean vst.variance vst.variance.expected vst.variance.standardized vst.variable feature_is_filtered feature_name feature_reference feature_biotype |
| schema_version title |
Tabula_Sapiens_Endothelial | tissue_in_publication assay_ontology_term_id donor_id anatomical_information n_counts_UMIs n_genes cell_ontology_class free_annotation manually_annotated compartment sex_ontology_term_id is_primary_data organism_ontology_term_id disease_ontology_term_id self_reported_ethnicity_ontology_term_id development_stage_ontology_term_id cell_type_ontology_term_id tissue_ontology_term_id suspension_type cell_type assay disease organism sex tissue self_reported_ethnicity development_stage | X_pca X_scvi X_scvi_umap X_umap | feature_type ensemblid highly_variable means dispersions dispersions_norm mean std feature_is_filtered feature_name feature_reference feature_biotype |
| _scvi _training_mode default_embedding dendrogram_cell_type_tissue dendrogram_computational_compartment_assignment dendrogram_consensus_prediction dendrogram_tissue_cell_type donor_id_colors donor_method_colors hvg method_colors neighbors schema_version sex_colors tissue_in_publication_colors title umap |
nf-core/checkatlas Methods Description
Suggested text and references to use when describing pipeline usage within the methods section of a publication.
Methods
Data was processed using nf-core/checkatlas v1.0dev of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.
The pipeline was executed with Nextflow v23.04.1 (Di Tommaso et al., 2017) with the following command:
nextflow run nf-core-checkatlas -r dev --path /data/analysis/data_becavin/checkatlas_test/tuto/data1/
References
- Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
- Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
- Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
- da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192
Notes:
- If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
- The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
- You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.
nf-core/checkatlas Software Versions
are collected at run time from the software output.
Process Name | Software | Version |
---|---|---|
CUSTOM_DUMPSOFTWAREVERSIONS | python | 3.10.12 |
yaml | 6.0.1 | |
LIST_CELLRANGER_ATLASES | checkatlas | 0.3.9 |
LIST_SCANPY_ATLASES | checkatlas | 0.3.9 |
LIST_SEURAT_ATLASES | checkatlas | 0.3.9 |
Workflow | Nextflow | 23.04.1 |
nf-core/checkatlas | 1.0dev |
nf-core/checkatlas Workflow Summary
- this information is collected when the pipeline is started.
Core Nextflow options
- revision
- dev
- runName
- stoic_pauling
- launchDir
- /data/analysis/data_becavin/checkatlas_test/tuto
- workDir
- /data/analysis/data_becavin/checkatlas_test/tuto/work
- projectDir
- /home/becavin/.nextflow/assets/becavin-lab/nf-core-checkatlas
- userName
- becavin
- profile
- standard
- configFiles
- N/A
Input/output options
- path
- /data/analysis/data_becavin/checkatlas_test/tuto/data1/
- outdir
- N/A